Glycosidases of Phaseolus vulgaris

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Glycosidases of Phaseolus vulgaris

The enzymes, cr-galactosidase, P-galactosidase, a-mannosidase, P-glucosidase, and p-acetylglucosaminidase, have been purified from the germinating seeds of Phaseolus vulgaris. All five enzymes have been simultaneously isolated in a highly active form. The pH optimum, K,,,, and energy of activation of each glycosidase for the reaction of hydrolysis of the appropriate P-nitrophenyl glycoside have...

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Oligosaccharide Specificities of Phaseolus vulgaris

The structural determinants required for interaction of oligosaccharides with leukoagglutinating phytohemagglutinin (L-PHA) and erythroagglutinating phytohemagglutinin (E-PHA) from Phaseolus vulgaris have been studied by immobilized lectin affinity chromatography. Homogeneous oligosaccharides of known structure, purified following release from Asn with Nglycanase and reduction with NaBH4, were ...

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Uromyces phaseoli and Phaseolus vulgaris

To study the function of bean rust haustoria either the host, Phaseolus vulgaris, or the parasite, Uromyces phaseoli, were labeled with 3H-lysine. 24h and 48 h after inoculation samples of the infected leaves were taken and processed for electronmicroscopic autoradiography. When the host was labeled, silver grain densities over the structures of host and parasite were compared. Very low grain d...

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Factors Affecting Root Formation of Phaseolus Vulgaris.

The successful rootingof leafy cuttings inivolves three physiologieally distinct processes: initiation of root primordia; development of true roots from these initials; and mainitenance of the cutting as ani excised organ during the rooting. The first of these, where it does niot take place sponitanieously, cani be brought about in many plants by treatment with auxini. The second (outgrow^th of...

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Retrograde Transport of the Lectin Phaseolus vulgaris

The transport properties of the leftin Phaseolus vulgaris leucoagglutinin (PHA-L) were tested h the frog central nervous system. After delivery of the lectin to the lower brainstem by iontophoresis, stained axons and axon terminals, as well as neurons with richly arborizing dendrites, were observed in different regions of the brain and spinal cord even far away from the site of application. The...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1968

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)99329-4